MOLECULAR BIOLOGY-SUPPLY 2K6

Code No: RR222305 Set No. 1
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What is the relationship between nucleosomes, 30 nm fibers and the scaffold struc-
ture with respect to the organization of DNA in the nucleus? [16]
2. Why is the 3’OH group on the ribose ring so important for DNA and RNA synthe-
sis? [16]
3. Eukaryotic mRNA is usually monocistronic. What does this mean? Why is this
the case for eukaryotes, whereas polycistronic mRNA is often found in prokaryotes?
[16]
4. Describe the following terms ribosome binding site, RNA polymerase, tRNA syn-
thetases. [16]
5. Write short notes on
(a) Thymine dimers
(b) Error prone Repair [16]
6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic? [16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. What are the differences among ans IS elements, a transposon, an intron and a
plasmid. [16]
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Code No: RR222305 Set No. 2
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What are the roles of RNA primers and Okazaki fragments during DNA replication?
[16]
2. Describe Meselson-Stahl experiment. [16]
3. Write the following
(a) RNA Pol “basal” factors
(b) .RNA Pol “Transcription factors”? [16]
4. Write briefly on the different types of protein that exists. [16]
5. What is significantly different about the type of DNA “damage” repaired by mis-
match repair compared to other types of DNA damage? [16]
6. Write a note on microbial testing that are widely used in industrial research.
[16]
7. What is the role of Rec protein in bacterial recombination? [16]
8. Describe lysis and lysogeny. [16]
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Code No: RR222305 Set No. 3
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What were the features of the DNA molecule described by Watson and Crick? Are
these features the same for all DNA molecules? [16]
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]
3. Write short notes on
(a) Rho dependent termination.
(b) Rho independent termination. [16]
4. What will determine whether regions of alpha-helical structure lie at the surface or
in the interior of a water-soluble globular protein? [16]
5. What is meant by Reverse Genetics? How is useful? [16]
6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic? [16]
7. With respect to F+ and F− bacterial mating, answer the followings
(a) How was it established that physical contact was necessary?
(b) How was it established that chromosome transfer was unidirectional?
[16]
8. What are the differences among ans IS elements, a transposon, an intron and a
plasmid. [16]
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Code No: RR222305 Set No. 4
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Summarize and compare the properties of DNA polymerase- I, II and III. [16]
2. In Meselson-Stahl experiment, which of the three modes of replication could be
ruled out after one round of replication? After two round? [16]
3. What processing events occur with prokaryotic mRNA? [16]
4. Which feature of the Genetic code is responsible for redundancy of Geretic Code.
[16]
5. What is significantly different about the type of DNA “damage” repaired by mis-
match repair compared to other types of DNA damage? [16]
6. Write a note on split genes. [16]
7. What is the role of Rec protein in bacterial recombination? [16]
8. Describe what are the three different physical forms of phage λ chromosome?
[16]
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