BIO-PROCESS ENGINEERING-I-REG 2K4

Code No: RR-222302
II-B.Tech. II-Semester Regular Examinations, April/May-2004
BIO-PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 Hours Max. Marks: 80
Answer any FIVE questions
All questions carry equal marks
- - -
1.a) What is chromatography? Explain the important chromatographic methods.
b) Draw the schematic diagram of a typical chromatography column and explain the
working principle giving the general material balance.
2.a) What is Baker’s yeast? Explain briefly the process for producing Baker’s yeast.
b) What is High-Fructose Corn syrup? What are the commercial uses? Describe the
process for producing high-fructose corn syrup.
3. Explain the important points to be considered for medium formulation.
4. What is meant by filter sterilization? Explain its application in media sterilization.
5. Explain the concept of Degree of Reduction and its application in proton-electron
balance in biorector.
6. Explain the transfer of bioenery via ATP.
7. Enumerate various environmental conditions that effect the growth kinetics.
8. Explain the substrate and product inhibition on the product formation with
appropriate examples.
^^^
Set No:
1
Code No: RR-222302
II-B.Tech. II-Semester Regular Examinations, April/May-2004
BIO-PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 Hours Max. Marks: 80
Answer any FIVE questions
All questions carry equal marks
- - -
1.a) What are the important parameters to be considered for selecting a solvent in
liquid-liquid extraction for inhibitory products?
b) Draw the flow diagram and write the material balance of a single stage extraction
system and derive an expression for extraction factor.
2.a) What are the commercial uses of acetone and butanol? Name the species and
substrates used for acetone-butanol production.
b) Draw the flow diagram for the process for acetone-butanol production. Name the
other fermentation products, and micronutrients used in the process.
3. Give the composition of four industrially important media used in industrial
fermentation.
4. Describe the methods for sterilizing air in aerobic fermentors.
5. Explain in detail the stoichiometry involved in the cell growth and product
formation.
6. Enumerate the aerobic catabolism of glucose with emphasis on energetics.
7. Enumerate in detail various environmental conditions that affect the growth
kinetics.
8. Briefly discuss about the structured models for product formation and compare
with growth.
^^^
Set No:
2
Code No: RR-222302
II-B.Tech. II-Semester Regular Examinations, April/May-2004
BIO-PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 Hours Max. Marks: 80
Answer any FIVE questions
All questions carry equal marks
- - -
1.a) Explain aqueous two phase extraction and its application in product recovery.
Give examples.
b) Describe adsorption giving the schematic flow diagram and explain the industrial
application.
2.a) Describe the process for ethanol production explaining the stoichiometry, names
of organisms, substrates, and process conditions.
b) Discuss the commercial uses of ethanol.
3. Describe the use of precursors and metabolic regulators in media preparation.
4.a) Describe the methods for sterilizing fermenters.
b) Explain the methods of sterilizing variety of additives administered during the
process of fermentation.
5. Discuss in detail the stoichiometry of the product formation with an example.
6. What are the major steps in aerobic metabolism of hydrocarbons? What are the
end products?
7. Explain specific growth rate with relevant equations.
8. Enumerate difference between growth and non-growth associated product
kinetics.
^^^
Set No:
3
Code No: RR-222302
II-B.Tech. II-Semester Regular Examinations, April/May-2004
BIO-PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 Hours Max. Marks: 80
Answer any FIVE questions
All questions carry equal marks
- - -
1.a) Draw a generalized flow sheet for fermentation process indicating the important
upstream and downstream sections.
b) Describe the factors for the cost analysis of a industrial fermentation process.
2.a) Describe the status and details of animal cell culture, the medium compositions,
process conditions, contaminants, and major products involved.
b) What is meant by plant tissue culture? Explain the commercial importance of
plant tissue culture giving examples of major products.
3. Describe in detail the theory of oxygen requirement and supply in industrial
fermentation.
4. An autoclave malfunctions, and the temperature reaches only 119.5 oC. The
sterilization time at the maximum temperature was 20 minutes. The jar contains
10 litre of complex medium that has 105 spores/lit. At 121 oC specific death rate
(kd) = 1.0 min-1 and activation energy for the death (E0d) = 90 kcals/g-mol. What
is the probability that the medium was sterile?
5. Enumerate the elemental material balance growth with an example.
6. Differentiate the heterotrophic and autotrophic metabolism emphasis on
energetics.
7. Discuss in detail the kinetics of microbial growth and substrate consumption.
8. Critically evaluate the application of Leudejking-Piret model in bioprocess
engineering.
^^^
Set No:
4