MOLECULAR BIOLOGY-REG 2K5

Code No: RR222305 Set No.1
II B.Tech. II Semester Regular Examinations, April/May -2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Write the detail components of DNA molecule that are organized inside cell.
2. compare conservative, semiconservative and dispersive modes of DNA replication.
3. How does the structure of a eukaryotic CoreRNAPol compare with
the E. coli CoreRNApol?
4. Discuss the roles of the following in protein expression: rRNA, tRNA, mRNA.
5. Write an essay that describes mutations.
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation.
7. Distinguish among the three modes of recombination in bacteria.
8. Describe what are the three different physical forms of phage  chromosome?
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Code No: RR222305 Set No.2
II B.Tech. II Semester Regular Examinations, April/May -2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. In what component parts do DNA and RNA differs?
2. Describe Meselson-Stahl experiment.
3. Write the following
(a) RNA Pol “basal” factors
(b) .RNA Pol “Transcription factors”?
4. Why would isoleucine be less likely to occur in a beta turn than in a beta strand?
5. Write an essay that describes mutations.
6. Write a note on molecular structure of eukaryotic gene.
7. Why are the recombinants produced from an HfrXF− cross never F+?
8. Describe the methods of transduction.
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Code No: RR222305 Set No.3
II B.Tech. II Semester Regular Examinations, April/May -2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. If the tetranucleotide hypothesis were correct regarding the simplicity of DNA
structure, under what circumstances could DNA be the genetic material.
2. What are two differences between DNA and RNA? What bases pair with each
other?
3. Where are each of the RNA polymerase types found in the eukaryotic cell?
4. Name and describe the 4 levels of protein structure?
5. Most mutations are thought to be deleterious. Why then, is it reasonable to state
that mutations are essential for evolutionary process?
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation.
7. What do you mean by plasmid? Describe their role in recombination.
8. Why are IS elements sometimes referred to as selfish DNA?
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Code No: RR222305 Set No.4
II B.Tech. II Semester Regular Examinations, April/May -2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. What are the roles of RNA primers and Okazaki fragments during DNA replica-
tion?
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional?
3. Write short notes on
(a) Rho dependent termination.
(b) Rho independent termination.
4. What will determine whether regions of alpha-helical structure lie at the surface or
in the interior of a water-soluble globular protein?
5. Describe different types of mutations; and explain the importance of mutations for
genetic research.
6. Which are the functional portions of a functional gene?
7. Describe the mechanism of transformation process.
8. What are Retroposons?
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