BIO PROCESS ENGINEERING-I-AUG 2K8

Code No: RR222302 Set No. 1
II B.Tech Supplimentary Examinations, Aug/Sep 2008
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Write shorts notes on:
(a) Microbial biomass
(b) Microbial enzymes. [8+8]
2. (a) What is aseptic operation and containment? [4]
(b) Describe a typical aseptic, aerobic fermentation process. [4]
(c) What is sparger? Describe different spargers used in fermentors. [2+6]
3. Sodium Bicarbonate is used as carbon source for the commercial production of
a micro algae. A constant cell density is always maintained in the reactor by
harvesting the cells daily. The growth rate is measured regularly and an average
growth rate of 0.1 gm/lit (dry weight) is recorded daily. The total culture volume
is 1100 litres. Sodium bicarbonate is the only Carbon source and it is added daily.
Assume that the cells are 50% Carbon by weight. Calculate the quantity of Sodium
Bicarbonate to be added daily if the conversion efficiency is assumed to be 90%.
[16]
4. (a) What are the advantages of continuous sterilisation.
(b) What are the advantages of batch sterilisation. [8+8]
5. Discuss the following:
(a) Stoichiometry of cell growth and product formation
(b) Degree of reduction of substrate and biomass. [8+8]
6. Differentiate the heterotrophic and autotrophic metabolism emphasis on energetics.
[16]
7. Define the following:
(a) Maximum growth rate
(b) Yield on substrate
(c) Mass doubling time
(d) Specific growth rate [4+4+4+4]
8. Explain the following with respect to product formation
(a) Substrate inhibition
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Code No: RR222302 Set No. 1
(b) Product inhibition. [8+8]
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Code No: RR222302 Set No. 2
II B.Tech Supplimentary Examinations, Aug/Sep 2008
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention about the Regulatory constraints of bioprocesses. [6+10]
2. (a) What is aseptic operation and containment? [4]
(b) Describe a typical aseptic, aerobic fermentation process. [4]
(c) What is sparger? Describe different spargers used in fermentors. [2+6]
3. Explain the factors to be considered for developing medium for animal cell culture.
[16]
4. Describe the design featurers of continuous sterilisation processes. [16]
5. Enumerate the difference in prediction of yield coefficients in anaerobic and anaer-
obic system with appropriate example. [16]
6. Briefly discuss the following:
(a) Energy capture efficiency
(b) Oxygen consumption and heat evolution in aerobic cultures
(c) Heat generation and yield factor estimation. [5+5+6]
7. Explain specific growth rate with relevant equations. [16]
8. Explain the following:
(a) Competitive product inhibition
(b) Non-competitive product inhibition. [8+8]
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Code No: RR222302 Set No. 3
II B.Tech Supplimentary Examinations, Aug/Sep 2008
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention about the Regulatory constraints of bioprocesses. [6+10]
2. Derive an expression for estimating the heat transfer area required to obtain ade-
quate temperature control in a fermentor. [16]
3. Sodium Bicarbonate is used as carbon source for the commercial production of
a micro algae. A constant cell density is always maintained in the reactor by
harvesting the cells daily. The growth rate is measured regularly and an average
growth rate of 0.1 gm/lit (dry weight) is recorded daily. The total culture volume
is 1100 litres. Sodium bicarbonate is the only Carbon source and it is added daily.
Assume that the cells are 50% Carbon by weight. Calculate the quantity of Sodium
Bicarbonate to be added daily if the conversion efficiency is assumed to be 90%.
[16]
4. Describe the design featurers of continuous sterilisation processes. [16]
5. Explain the concept of Degree of Reduction and its application in proton-electron
balance in biorector. [16]
6. (a) Discuss the role of control sites in aerobic glucose metabolism
(b) Oxygen consumption and heat evolution in aerobic cultures. [8+8]
7. Give short note on:
(a) Lag phase
(b) Logarithmic phase
(c) Stationary phase
(d) Death phase. [4+4+4+4]
8. Explain the optimum environmental conditions required for growth and product
formation. [16]
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Code No: RR222302 Set No. 4
II B.Tech Supplimentary Examinations, Aug/Sep 2008
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention different types of enzymes extracted from plant and their application.
[8+8]
2. (a) Explain the major components of a chemostat with the help of a diagram
giving the notations used in modeling and analysis.
(b) Explain CSTR with recycle using a schematic diagram.
(c) Describe ideal plug flow tubular reactor giving notations used for analysis and
modeling. [6+6+4]
3. Describe in detail the theory of oxygen requirement and supply in industrial
fermentation. [16]
4. Explain the kinetics of medium sterilisation and obtain a mathematical expression
for specific death rate. [16]
5. Explain in detail the stoichiometry involved in the cell growth and product forma-
tion. [16]
6. Enumerate the aerobic catabolism of glucose with emphasis on energetics. [16]
7. (a) Enumerate the principle involved in the microbial growth taking an example.
(b) Differentiate between the growth in the batch and continuos systems. [8+8]
8. Give brief notes on structured models for growth and product formation with rel-
evant examples. [16]
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