BIO PROCESS ENGINEERING-I-REG 2K6

Code No: RR222302 Set No. 1
II B.Tech II Semester Regular Examinations, Apr/May 2006
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Mention about the Regulatory constraints of bioprocesses. 16 [6+10]
2. (a) Briefly explain about the material of construction for fermentor fabrication.
[8]
(b) Explain different types of agitators used in fermentors. [8]
3. (a) What are the major sources of carbon, nitrogen and phosphorous in industrial
fermentation. [8]
(b) What are chelating agents? Explain their functions giving example. [2+6]
4. (a) Explain reactions involved that lead to loss of nutrient quality during sterili-
sation. [8]
(b) Describe methods of batch sterilisation. [8]
5. Discuss the following:
(a) Medium formulation [8]
(b) Yield factor. [8]
6. Explain the synthesis of glucose from pyruvate. [16]
7. Give short note on:
(a) Lag phase [4]
(b) Logarithmic phase [4]
(c) Stationary phase [4]
(d) Death phase. [4]
8. Explain the difference between:
(a) Competitive and non-competitive product inhibition [8]
(b) Growth and non-growth associated products. [8]
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Code No: RR222302 Set No. 2
II B.Tech II Semester Regular Examinations, Apr/May 2006
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention different types of enzymes extracted from plant and their application.
[8+8]
2. (a) Briefly explain about the material of construction for fermentor fabrication.
[8]
(b) Explain different types of agitators used in fermentors. [8]
3. Explain the factors influencing the choice of carbon source. [16]
4. Derive the mathematical expression for Nutrient Quality Criterion (Q) in continu-
ous sterilisation and describe the graphical method to optimise temperature-time
regime. [16]
5. Discuss the following in detail
(a) Stoichiometricallly limiting compounds [8]
(b) Growth rate limiting medium. [8]
6. Briefly discuss the following:
(a) Energy capture efficiency [5]
(b) Oxygen consumption and heat evolution in aerobic cultures [5]
(c) Heat generation and yield factor estimation. [6]
7. Give note on the following:
(a) Substrate limited growth [6]
(b) Unbalanced growth [5]
(c) Balanced Growth. [5]
8. Describe growth associated product formation with equation. [16]
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Code No: RR222302 Set No. 3
II B.Tech II Semester Regular Examinations, Apr/May 2006
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention about the Regulatory constraints of bioprocesses. 16 [6+10]
2. What is meant by solid state fermentation? Explain the industrial application of
solid state fermentation indicating the microorganisms, substrates and products.
[6+10]
3. (a) Explain the use of water as an important constituent for fermentation. [8]
(b) Describe the use of buffers for media preparation in fermentation. [8]
4. Describe the design featurers of continuous sterilisation processes. [16]
5. Determine the yield coefficients (YX/SandYX/02) and total amount of oxygen re-
quired in a batch reactor of 10000 liters volume with the growth of yeast on glucose
as per the equation given
C6H12O6+3O2+0.48NH3− − − − −− ! 0.48C6H10N03+4.32H2O + 3.12CO2
Final yeast concentration of 47 gdw/l is required. [16]
6. Differentiate the heterotrophic and autotrophic metabolism emphasis on energetics.
[16]
7. (a) Enumerate the difference between the cell growth in batch and continuos cul-
tures [8]
(b) Explain the kinetics of microbial growth. [8]
8. Give brief notes on structured models for growth and product formation with rel-
evant examples. [16]
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Code No: RR222302 Set No. 4
II B.Tech II Semester Regular Examinations, Apr/May 2006
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Write a short notes on:
(a) Transformation process [8]
(b) Microbial metabolites. [8]
2. In a fed batch culture operating with an intermittent addition of glucose solution,
values of the following parameters are given at time t =2hours, when the system is
at quasi steady state. Culture volume (V) = 1 litre Substrate concentration (S0) =
100 gm glucose / litre Limiting rate constant (KS) = 0.1 gm glucose / litre Initial
amount of biomass in the reactor (Xt
0) = 30 gm Nutrient feed flow rate (F) = dv/dt
= 200 ml/hour Maximum specific growth rate = 0.3 h−1 Yield coefficient (YM
x/s) =
0.5 gm cells / gm glucose
Determine:
(a) the initial culture of the medium (V0). [4]
(b) Determine the concentration of the growth limiting substrate in the vessel at
quasi steady state. [4]
(c) Determine the concentration and total amount of biomass in the vessel at t
=2 hour (at quasi steady state). [4]
(d) If specific rate of product formation (qp) = 0.2 gm product/cells, P0= 0 de-
termine the concentration of the product in the vessel at t=2 hour. [4]
3. (a) Explain the use of water as an important constituent for fermentation. [8]
(b) Describe the use of buffers for media preparation in fermentation. [8]
4. Describe Arrhenius plot for the calculation of activation energy and derive an
expression for heat sterilisation of a pure culture at a constant temperature. [16]
5. Determine the degree of reductions for the substrate, bacteria, RQ and yield co-
efficients for aerobic degradation of an organic compound by a mixed culture of
organisms in wastewater as represented by the following reaction
C3H6O3+aO2+bNH3−− ! cC5H7NO2+dH2O + eCO2
Determine a, b, c, d and e, if YX/S = 0.4 g X/g S [16]
6. Differentiate the major function of the dark and light phases in photosynthesis.[16]
7. (a) Enumerate the principle involved in the microbial growth taking an example.
[8]
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Code No: RR222302 Set No. 4
(b) Differentiate between the growth in the batch and continuos systems. [8]
8. Describe growth and non-growth associated products formation with equations.
[16]
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