MOLECULAR BIOLOGY-SUPPLY 2K5

Code No: RR222305 Set No. 1
II B.Tech II Semester Supplementary Examinations,
November/December 2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Describe the various characteristics of the Watson-Crick DNA model. [16]
2. What are the requirements for the in vitro synthesis of DNA under the direction
of DNA polymerase-I? [16]
3. What is a “TATA box” and what is its function? Do all promoters have TATA
boxes? Why or why not? What provides the function of the TATA box when it
isn’t present? [16]
4. Describe the following terms: Transcription, translation, promoter. [16]
5. Describe different types of mutations; and explain the importance of mutations for
genetic research. [16]
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation. [16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. Write a note on Virus - mediated bacterial DNA transfer. [16]
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Code No: RR222305 Set No. 2
II B.Tech II Semester Supplementary Examinations,
November/December 2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What are the roles of RNA primers and Okazaki fragments during DNA replication?
[16]
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]
3. Where are each of the RNA polymerase types found in the eukaryotic cell? [16]
4. What is footprinting? How did it help define promoter sequences? [16]
5. What are mutagens? Classify the radiation, chemical mutagens that are affecting
the organisms. [16]
6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic? [16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. Why are IS elements sometimes referred to as selfish DNA? [16]
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Code No: RR222305 Set No. 3
II B.Tech II Semester Supplementary Examinations,
November/December 2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Summarize and compare the properties of DNA polymerase- I, II and III. [16]
2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]
3. What processing events occur with prokaryotic mRNA? [16]
4. Argue why the genetic code must be read 3 bases at a time rather than 2 bases.
[16]
5. Write short notes on
(a) Thymine dimers
(b) Error prone Repair [16]
6. Write a note of Benzer’s fine structure analysis of the rII locus of T4 bacteriophage.
[16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. What are the properties of viruses? How do viruses differ from all type of cells?
Why are viruses called obligate intracellular parasites? [16]
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Code No: RR222305 Set No. 4
II B.Tech II Semester Supplementary Examinations,
November/December 2005
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Describe the various characteristics of the Watson-Crick DNA model. [16]
2. What are two differences between DNA and RNA? What bases pair with each
other? [16]
3. Where are each of the RNA polymerase types found in the eukaryotic cell? [16]
4. Write a note on post-transnational modification. [16]
5. Describe different types of mutations; and explain the importance of mutations for
genetic research. [16]
6. Which are the functional portions of a functional gene? [16]
7. Describe the basis for chromosome mapping in the Hfr × F− crosses. [16]
8. Describe the mechanism of transduction process. [16]
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