MOLECULAR BIOLOGY-supply 2k4

Code No: RR-222305
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1. In what component parts do DNA and RNA differ?
2. What did the Watson-Crick model suggest about the replication of the DNA?
3. What is the process of removal of introns from pre-mRNA called? Where does it
occur? Where do other eukayrotic mRNA modifications take place?
4. Describe the functions of signal sequences and stop transfer sequences in the
export of proteins from the cytoplasm.
5. What is the role of the "DNA template" in excision repair? What is this
"template"?
6. Describe the Ames assay for screening potential environmental mutagens. Why is
it though that a compound that tests positively in the Ames assay may also be
carcinogenic?
7. Write a note on genetic recombination in Bacteria.
8. Describe the Shapiro model of transposition. What are the roles of transposase,
DNA polymeraseI, Ligase and resolvase?
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Set No.
1
Code No: RR-222305
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1. Sketch the shape of B-DNA and Z-DNA and write their properties.
2. Compare the conservative, semi-conservative, and dispersive modes of DNA
replication.
3. What is splicing and alternative splicing? Give examples of different types of
alternative splicing.
4. Explain the difference between unregulated protein production, transcriptional
regulation, translational regulation, and post-translational regulation.
5. Which of the proteins is an ATPase? What is an ATPase? Why is an ATPase
needed in excision repair?
6. Write a note on microbial testing that are widely used in industrial research.
7. Describe the conjugation process in F+ and F- bacteria.
8. How do transpons induce deletion and inversion?
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Set No.
2
Code No: RR-222305
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1. Describe the various characteristics of the Watson-Crick DNA model.
2. Describe Meselson-Stahl experiment.
3. What is hetergeneous nuclear RNA? How is it related to eukaryotic mRNA?
4. Describe the following terms: Transcription, translation, promoter.
5. What is the primary difference between transcription-coupled repair in
prokaryotes and in eukaryotes?
6. A point mutation occurs in a particular gene. Describe the types of mutational
events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation.
7. How do you map the chromosome in Hfr bacteria?
8. Describe how a transposon controls the expression of the flagellar phase in
salmonella.
*$*$*$*
Set No.
3
Code No: RR-222305
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1. What the physical state of DNA following denaturation?
2. In Meselson-Stahl experiment, which of the three modes of replication could be
ruled out
a) After one round of replication.
b) After two round.
3. What are the 5 general mechanisms used in RNA splicing? Explain each of them.
4. Describe the following terms ribosome binding site, RNA polymerase, tRNA
synthetases.
5. What is significantly different about the type of DNA "damage" repaired by
mismatch repair compared to other types of DNA damage?
6. Write a note of Benzer's fine structure analysis of the rII locus of T4
bacteriophage.
7. Describe the recombination process in F+ and F- mating.
8. Describe what are the three different physical forms of phage l chromosome.
*$*$*$*
Set No.
4