BIO PROCESS ENGINEERING-SUPPLY 2K8

Code No: R05222301 Set No. 1
II B.Tech Supplimentary Examinations, Aug/Sep 2008
BIO PROCESS ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Mention about different component parts of a fermentation process. [4+12]
2. (a) What is aseptic operation and containment? [4]
(b) Describe a typical aseptic, aerobic fermentation process. [4]
(c) What is sparger? Describe different spargers used in fermentors. [2+6]
3. Explain the factors to be considered for developing medium for animal cell culture.
[16]
4. (a) What are the important information required for the design of batch sterili-
sation process. [8]
(b) Define Del factor. Describe the calculation of Del factor during heating and
cooling. [2+6=8]
5. Determine the yield coefficients (YX/SandYX/02) and total amount of oxygen re-
quired in a batch reactor of 10000 liters volume with the growth of yeast on glucose
as per the equation given
C6H12O6+3O2+0.48NH3− − − − −− ! 0.48C6H10N03+4.32H2O + 3.12CO2
Final yeast concentration of 47 gdw/l is required. [16]
6. Discuss about the respiration chain and the electron transport along the respiratory
chain. [16]
7. (a) Enumerate the difference between the cell growth in batch and continuos cul-
tures
(b) Explain the kinetics of microbial growth. [8+8]
8. Discuss in details the product kinetics associated with growth with appropriate
examples. [16]
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Code No: R05222301 Set No. 2
II B.Tech Supplimentary Examinations, Aug/Sep 2008
BIO PROCESS ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention about the Regulatory constraints of bioprocesses. [6+10]
2. What is meant by solid state fermentation? Explain the industrial application of
solid state fermentation indicating the microorganisms, substrates and products.
[6+10]
3. (a) Explain the use of water as an important constituent for fermentation.
(b) Describe the use of buffers for media preparation in fermentation. [8+8]
4. Derive the mathematical expression for Nutrient Quality Criterion (Q) in continu-
ous sterilisation and describe the graphical method to optimise temperature-time
regime. [16]
5. Determine coefficients a, b, c and d (where RQ=0.66) along with the biomass yield
coefficient and oxygen yield coefficient for aerobic degradation of benzoic acid by a
mixed culture of microorganisms as represented by the following overall reaction
C6H5COOH + aO2+bNH3−− ! cC5H7NO2+dH2O + eCO2 [16]
6. Enumerate the aerobic catabolism of glucose with emphasis on energetics. [16]
7. (a) Explain the procedure involved in the determination of cell number density
and cell mass concentration.
(b) Give a short note on simple unstructured kinetic models for microbial growth.
[8+8]
8. Describe how the microbial products can be classified along with the equations.
[16]
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Code No: R05222301 Set No. 3
II B.Tech Supplimentary Examinations, Aug/Sep 2008
BIO PROCESS ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Discuss in detail different unit operations used in manufacture of enzymes. [16]
2. What is meant by solid state fermentation? Explain the industrial application of
solid state fermentation indicating the microorganisms, substrates and products.
[6+10]
3. Describe in detail the theory of oxygen requirement and supply in industrial
fermentation. [16]
4. Describe Arrhenius plot for the calculation of activation energy and derive an
expression for heat sterilisation of a pure culture at a constant temperature. [16]
5. Enumerate the difference in prediction of yield coefficients in anaerobic and anaer-
obic system with appropriate example. [16]
6. Explain the transfer of bioenery via ATP. [16]
7. (a) Explain the procedure involved in the determination of cell number density
and cell mass concentration.
(b) Give a short note on simple unstructured kinetic models for microbial growth.
[8+8]
8. Explain the optimum environmental conditions required for growth and product
formation. [16]
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Code No: R05222301 Set No. 4
II B.Tech Supplimentary Examinations, Aug/Sep 2008
BIO PROCESS ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Discuss in detail about different commercial enzymes and its applications. [8+8]
2. In a fed batch culture operating with an intermittent addition of glucose solution,
values of the following parameters are given at time t =2hours, when the system is
at quasi steady state. Culture volume (V) = 1 litre Substrate concentration (S0) =
100 gm glucose / litre Limiting rate constant (KS) = 0.1 gm glucose / litre Initial
amount of biomass in the reactor (Xt
0) = 30 gm Nutrient feed flow rate (F) = dv/dt
= 200 ml/hour Maximum specific growth rate = 0.3 h−1 Yield coefficient (YM
x/s) =
0.5 gm cells / gm glucose
Determine:
(a) the initial culture of the medium (V0).
(b) Determine the concentration of the growth limiting substrate in the vessel at
quasi steady state.
(c) Determine the concentration and total amount of biomass in the vessel at t
=2 hour (at quasi steady state).
(d) If specific rate of product formation (qp) = 0.2 gm product/cells, P0= 0 deter-
mine the concentration of the product in the vessel at t=2 hour. [4+4+4+4]
3. Explain the factors to be considered for developing medium for animal cell culture.
[16]
4. (a) What are the consequences if a foreign microorganism invade a fermentation
process.
(b) Explain the methods to avoid this contamination. [8+8]
5. Discuss the following in detail
(a) Stoichiometricallly limiting compounds
(b) Growth rate limiting medium. [8+8]
6. What are the major steps in aerobic metabolism of hydrocarbons? What are the
end products? [16]
7. Explain the difference between the aerobic and anaerobic growth. [16]
8. Give brief notes on structured models for growth and product formation with rel-
evant examples. [16]
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