BIO PROCESS ENGINEERING-I-SUPPLY 2K6

Code No: RR222302 Set No. 1
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Mention about the Regulatory constraints of bioprocesses. 16 [6+10]
2. (a) What is aseptic operation and containment? [4]
(b) Describe a typical aseptic, aerobic fermentation process. [4]
(c) What is sparger? Describe different spargers used in fermentors. [2+6]
3. Sodium Bicarbonate is used as carbon source for the commercial production of
a micro algae. A constant cell density is always maintained in the reactor by
harvesting the cells daily. The growth rate is measured regularly and an average
growth rate of 0.1 gm/lit (dry weight) is recorded daily. The total culture volume
is 1100 litres. Sodium bicarbonate is the only Carbon source and it is added daily.
Assume that the cells are 50% Carbon by weight. Calculate the quantity of Sodium
Bicarbonate to be added daily if the conversion efficiency is assumed to be 90%.
[16]
4. (a) What are the consequences if a foreign microorganism invade a fermentation
process. [8]
(b) Explain the methods to avoid this contamination. [8]
5. Determine the yield coefficients (YX/SandYX/02) and total amount of oxygen re-
quired in a batch reactor of 10000 liters volume with the growth of yeast on glucose
as per the equation given
C6H12O6+3O2+0.48NH3− − − − −− ! 0.48C6H10N03+4.32H2O + 3.12CO2
Final yeast concentration of 47 gdw/l is required. [16]
6. Differentiate the major function of the dark and light phases in photosynthesis.[16]
7. Explain the following:
(a) Oxygen uptake rate [5]
(b) Critical oxygen concentration [5]
(c) Specific rate of oxygen consumption. [6]
8. Discuss in details the product kinetics associated with growth with appropriate
examples. [16]
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Code No: RR222302 Set No. 2
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Write Notes on:
(a) Biosensors [8]
(b) Biopesticides. [8]
2. (a) Briefly explain about the material of construction for fermentor fabrication.
[8]
(b) Explain different types of agitators used in fermentors. [8]
3. (a) Explain the use of water as an important constituent for fermentation. [8]
(b) Describe the use of buffers for media preparation in fermentation. [8]
4. (a) Describe the methods for sterilizing fermenters. [8]
(b) Explain the methods of sterilizing variety of additives administered during the
process of fermentation. [8]
5. Discuss the following in detail
(a) Stoichiometricallly limiting compounds [8]
(b) Growth rate limiting medium. [8]
6. Explain the synthesis of glucose from pyruvate. [16]
7. Explain growth pattern and kinetics in a batch culture. [8+8]
8. Explain the following with respect to product formation
(a) Substrate inhibition [8]
(b) Product inhibition. [8]
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Code No: RR222302 Set No. 3
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention about the Regulatory constraints of bioprocesses. 16 [6+10]
2. What is meant by solid state fermentation? Explain the industrial application of
solid state fermentation indicating the microorganisms, substrates and products.
[6+10]
3. Sodium Bicarbonate is used as carbon source for the commercial production of
a micro algae. A constant cell density is always maintained in the reactor by
harvesting the cells daily. The growth rate is measured regularly and an average
growth rate of 0.1 gm/lit (dry weight) is recorded daily. The total culture volume
is 1100 litres. Sodium bicarbonate is the only Carbon source and it is added daily.
Assume that the cells are 50% Carbon by weight. Calculate the quantity of Sodium
Bicarbonate to be added daily if the conversion efficiency is assumed to be 90%.
[16]
4. Explain the kinetics of medium sterilisation and obtain a mathematical expression
for specific death rate. [16]
5. (a) Describe the process of product formation with appropriate example. [8]
(b) Explain the maintenance coefficient with example. [8]
6. (a) Explain the thermodynamic efficiency of growth [8]
(b) Differentiate respiration and fermentation. [8]
7. (a) Enumerate the principle involved in the microbial growth taking an example.
[8]
(b) Differentiate between the growth in the batch and continuos systems. [8]
8. (a) Differentiate between competitive product inhibition and non-competitive prod-
uct inhibition. [8]
(b) Explain the product inhibition on the product formation. [8]
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Code No: RR222302 Set No. 4
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What do you mean be fermentation and range of Fermentation process? [4+12]
2. (a) What is meant by immobilisation of cells? What are the advantages of immo-
bilised cell culture over suspension cell culture? [2+4]
(b) What is active immobilisation? Explain various matrices used for active
immobilisation. [2+4]
(c) Describe the methods employed for active cell immobilisation. [4]
3. Describe in detail the theory of oxygen requirement and supply in industrial
fermentation. [16]
4. (a) What are the important information required for the design of batch sterili-
sation process. [8]
(b) Define Del factor. Describe the calculation of Del factor during heating and
cooling. [2+6=8]
5. Determine the rate of oxygen consumption and yield coefficients if rate of growth
at exponential phase is rx = 0.7 gdw/l-h in a batch reactor of 5000 liters volume
with the growth of yeast on glucose as per the equation given
C6H12O6+3O2+0.48NH3−− ! 0.48C6H10N03+4.32H2O + 3.12CO2
Final yeast concentration of 50 gdw/l is required. [16]
6. Differentiate the heterotrophic and autotrophic metabolism emphasis on energetics.
[16]
7. (a) Explain the procedure involved in the determination of cell number density
and cell mass concentration. [8]
(b) Give a short note on simple unstructured kinetic models for microbial growth.
[8]
8. Give brief notes on structured models for growth and product formation with rel-
evant examples. [16]
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