BIOPROCESS ENGINEERING-I-SUPPLY 2K4

Code No: RR-222302
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
BIOPROCESS ENGINEERING-I
(Bio Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1.a) Explain the common features of eucaryotic cell.
b) Describe the organisation and functions of procaryotic cell.
2.a) Explain activated sludge process with the schematic diagram and notations.
b) Describe anaerobic digestion using a flow diagram indicating various stages
and microbes involved in the process.
3. Explain the factors influencing the choice of carbon source.
4. Explain the kinetics of medium sterilisation and obtain a mathematical
expression for specific death rate.
5. Discuss the following with examples.
a) Growth yield coefficient
b) Product yield coefficient
6.a) Explain in detail the heat and energy in metabolic reactions.
b) Give a brief note on Crabtree effect.
7. Differentiate pH and redox potential and enumerate the role of pH and redox
potential on the growth kinetics.
8. Describe how the microbial products can be classified along with the
equations.
= + = + =
Set No.
1
Code No: RR-222302
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
BIOPROCESS ENGINEERING-I
(Bio Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1.a) What are enzymes? Briefly describe enzyme nomenclature.
b) Explain the medical and industrial utilization of enzymes giving the list of
industrially important enzymes, their source and application.
2.a) Draw the schematic diagrams of bubble column reactor and air loop reactor
and explain the working and industrial applications.
b) What is meant by fluidisation? Using schematic diagram explain packed bed
and fluidised bed biofilm reactors?
3.a) Describe the use of antifoam in industrial fermentation indicating the
principle.
b) Give examples of antifoam agents used in fermentation industry.
4. Describe Arrhenius plot for the calculation of activation energy and derive an
expression for heat sterilisation of a pure culture at a constant temperature.
5.a) Explain the available electron balances with appropriate examples.
b) Explain various yield coefficients of biomass and product formation by taking
an example.
6. Explain the following terms
a) Transamination
b) Energy and heat in metabolic reactions
c) Energy capture efficiency.
7.a) Enumerate the difference between the cell growth in batch and continuos
cultures.
b) Explain the kinetics of microbial growth.
8. Describe growth associated product formation with equation.
= + = + =
Set No.
2
Code No: RR-222302
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
BIOPROCESS ENGINEERING-I
(Bio Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1.a) Compare protozoa with algae in terms of their cellular structure and functions.
b) Explain the major biological functions of proteins.
2. Derive an expression for estimating the heat transfer area required to obtain
adequate temperature control in a fermentor.
3. What are the factors influencing the choice of nitrogen source?
4. Describe the design features of continuous sterilisation processes.
5.a) Describe the process of product formation with appropriate example.
b) Explain the maintenance coefficient with example.
6. Discuss about the partial oxidation and its end products with an example.
7. Explain the difference between the aerobic and anaerobic growth.
8. Give brief notes on structured models for growth and product formation with
relevant examples.
= + = + =
Set No.
3
Code No: RR-222302
II B.Tech. II-Semester Supplementary Examinations, Nov/Dec-2004
BIOPROCESS ENGINEERING-I
(Bio Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
- - -
1.a) Draw a schematic diagram of continuous rotary vacuum filter and explain the
working principle.
b) Write a note on coagulation and flocculation.
2. Give the material balance in a chemostat with recycle and derive an expression
for the cell concentration in the recycle stream.
3.a) Explain the use of water as an important constituent for fermentation.
b) Describe the use of buffers for media preparation in fermentation.
4. Derive the mathematical expression for Nutrient Quality Criterion (Q) in
continuous sterilisation and describe the graphical method to optimise
temperature-time regime.
5. Enumerate the difference between the stoichiometry of cell growth and
product formation.
6. Briefly specify major function of the TCA cycle.
7. Explain Monod model application in the bioprocess engineering along with
applications.
8. Describe growth and non-growth associated products formation with
equations.
= + = + =
Set No.
4