BIO PROCESS ENGINEERING-I-REG 2K5

Code No: RR222302 Set No.1
II B.Tech. II Semester Regular Examinations, April/May -2005
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Discuss in detail about various microorganisms used as biopesticides
2. After a batch fermentation, the system is dismantled and approximately 75% of
the cell mass is suspended in the liquid phase (2 litre), while 25% is attached
to the reactor walls and internals as a thick film. Work with radioactive tracers
shows that 50% of the target product (intracellular) is associated with each cell
fraction. The productivity of this reaction is 2 gm product per litre at the 2 litre
scale. What would be the productivity at 20,000 litre scale if both reactors had a
height-diameter ratio of 2 to 1.
3. Explain the factors to be considered for developing medium for animal cell culture.
4. Describe Arrhenius plot for the calculation of activation energy and derive an
expression for heat sterilisation of a pure culture at a constant temperature.
5. Explain the concept of Degree of Reduction and its application in proton-electron
balance in biorector.
6. Discuss about the partial oxidation and its end products with an example.
7. (a) Enumerate the difference between the cell growth in batch and continuos cul-
tures
(b) Explain the kinetics of microbial growth
8. Explain the substrate and product inhibition on the product formation with
appropriate examples.
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Code No: RR222302 Set No.2
II B.Tech. II Semester Regular Examinations, April/May -2005
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Mention about the Regulatory constraints of bioprocesses.
2. (a) What is aseptic operation and containment?
(b) Describe a typical aseptic, aerobic fermentation process.
(c) What is sparger? Describe different spargers used in fermentors.
3. Determine the amount of (NH4)2SO4 to be supplied in a fermentation medium
where the final cell concentration is 30 gm/lit in a 1000 lit culture volume. Assume
that cells are 12% Nitrogen by weight and (NH4)2SO4 is the only Nitrogen source.
4. (a) What are the important information required for the design of batch sterili-
sation process.
(b) Define Del factor. Describe the calculation of Del factor during heating and
cooling.
5. Discuss the concept of Elemental Balances with example using simplified biological
conversion.
6. Briefly discuss the following
(a) Energy capture efficiency
(b) Oxygen consumption and heat evolution in aerobic cultures
(c) Heat generation and yield factor estimation
7. Enumerate in detail various environmental conditions that affect the growth kinet-
ics
8. Explain the difference between:
(a) Competitive and non-competitive product inhibition
(b) Growth and non-growth associated products
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Code No: RR222302 Set No.3
II B.Tech. II Semester Regular Examinations, April/May -2005
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. What do you mean by down stream processes explain with flow chart.
2. Derive an expression for estimating the heat transfer area required to obtain ade-
quate temperature control in a fermentor.
3. (a) Explain the use of water as an important constituent for fermentation.
(b) Describe the use of buffers for media preparation in fermentation.
4. (a) What are the consequences if a foreign microorganism invade a fermentation
process.
(b) Explain the methods to avoid this contamination.
5. Determine the rate of oxygen consumption and yield coefficients if rate of growth
at exponential phase is rx = 0.7 gdw/l-h in a batch reactor of 5000 liters volume
with the growth of yeast on glucose as per the equation given
C6H12O6+3 O2+0.48NH3−− ! 0.48C6H10N03+4.32H2O + 3.12CO2
Final yeast concentration of 50 gdw/l is required.
6. Given an overview of
(a) Anaerobic and aerobic metabolism
(b) Oxygen consumption and heat evolution in aerobic cultures
7. (a) Explain the procedure involved in the determination of cell number density
and cell mass concentration
(b) Give a short note on simple unstructured kinetic models for microbial growth.
8. Explain the difference between:
(a) Competitive and non-competitive product inhibition
(b) Growth and non-growth associated products
? ? ? ? ?
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Code No: RR222302 Set No.4
II B.Tech. II Semester Regular Examinations, April/May -2005
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Draw and Explain a typical fermentation process.
2. (a) What is aseptic operation and containment?
(b) Describe a typical aseptic, aerobic fermentation process.
(c) What is sparger? Describe different spargers used in fermentors.
3. Determine the amount of (NH4)2SO4 to be supplied in a fermentation medium
where the final cell concentration is 30 gm/lit in a 1000 lit culture volume. Assume
that cells are 12% Nitrogen by weight and (NH4)2SO4 is the only Nitrogen source.
4. (a) What are the important information required for the design of batch sterili-
sation process.
(b) Define Del factor. Describe the calculation of Del factor during heating and
cooling.
5. Determine coefficients a, b, c and d (where RQ=0.66) along with the biomass yield
coefficient and oxygen yield coefficient for aerobic degradation of benzoic acid by a
mixed culture of microorganisms as represented by the following overall reaction
C6H5COOH + aO2+bNH3−− ! cC5H7NO2+dH2O + eCO2
6. Given an overview of
(a) Anaerobic and aerobic metabolism
(b) Oxygen consumption and heat evolution in aerobic cultures
7. Briefly explain the following
(a) Steady-sate biomass concentration
(b) Specific rate of the oxygen consumption
8. Give a short notes on the product kinetics of
(a) Growth associated (primary)
(b) Non-growth associated (secondary)
? ? ? ? ?
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