BIO PROCESS ENGINEERING-I-SUPPLY 2K7

Code No: RR222302 Set No. 1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention about the Regulatory constraints of bioprocesses. [6+10]
2. (a) Briefly explain about the material of construction for fermentor fabrication.
(b) Explain different types of agitators used in fermentors. [8+8]
3. Describe the use of precursors and metabolic regulators in media preparation.[16]
4. (a) Explain reactions involved that lead to loss of nutrient quality during sterili-
sation.
(b) Describe methods of batch sterilisation. [8+8]
5. Discuss the concept of Elemental Balances with example using simplified biological
conversion. [16]
6. Briefly specify major function of the TCA cycle. [16]
7. (a) Enumerate the principle involved in the microbial growth taking an example.
(b) Differentiate between the growth in the batch and continuos systems. [8+8]
8. Explain the difference between:
(a) Competitive and non-competitive product inhibition
(b) Growth and non-growth associated products. [8+8]
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Code No: RR222302 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention different types of enzymes extracted from plant and their application.
[8+8]
2. (a) What is meant by immobilisation of cells? What are the advantages of immo-
bilised cell culture over suspension cell culture? [2+4]
(b) What is active immobilisation? Explain various matrices used for active
immobilisation. [2+4]
(c) Describe the methods employed for active cell immobilisation. [4]
3. Describe the use of precursors and metabolic regulators in media preparation.[16]
4. (a) Draw the schematic diagram of a typical continuous injector- flash cooler ster-
iliser.
(b) Draw the flow diagram of continuous strilisation system employing spiral heat
exchangers. [8+8]
5. Estimate the theoretical growth and product yield coefficients for ethanol fermen-
tation by S cerevisiae as described by the following reaction
C6H12O6
− − − − − − −− ! 2C2H5OH + 2CO2 [16]
6. Explain the following:
(a) Control sites in metabolism [8]
(b) Transfer of bioenergy via ATP. [8]
7. Explain Monod model application in the bioprocess engineering along with
applications. [16]
8. (a) Differentiate between competitive product inhibition and non-competitive prod-
uct inhibition.
(b) Explain the product inhibition on the product formation. [8+8]
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Code No: RR222302 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What do you mean be fermentation and range of Fermentation process? [4+12]
2. (a) What is aseptic operation and containment? [4]
(b) Describe a typical aseptic, aerobic fermentation process. [4]
(c) What is sparger? Describe different spargers used in fermentors. [2+6]
3. Describe the use of precursors and metabolic regulators in media preparation.[16]
4. What is meant by filter sterilization? Explain its application in media sterilization.
[4+12]
5. Discuss the following:
(a) Medium formulation
(b) Yield factor. [8+8]
6. (a) Explain in detail the heat and energy in metabolic reactions.
(b) Give a brief note on Crabtree effect. [8+8]
7. (a) Enumerate the principle involved in the microbial growth taking an example.
(b) Differentiate between the growth in the batch and continuos systems. [8+8]
8. Explain the substrate and product inhibition on the product formation with
appropriate examples. [16]
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Code No: RR222302 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIO PROCESS ENGINEERING-I
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Mention about the Regulatory constraints of bioprocesses. [6+10]
2. (a) Briefly explain about the material of construction for fermentor fabrication.
(b) Explain different types of agitators used in fermentors. [8+8]
3. Give the composition of four industrially important media used in industrial fer-
mentation. [16]
4. Explain the kinetics of medium sterilisation and obtain a mathematical expression
for specific death rate. [16]
5. Discuss the following:
(a) Stoichiometry of cell growth and product formation
(b) Degree of reduction of substrate and biomass. [8+8]
6. Explain the synthesis of glucose from pyruvate. [16]
7. (a) Enumerate the principle involved in the microbial growth taking an example.
(b) Differentiate between the growth in the batch and continuos systems. [8+8]
8. (a) Differentiate between competitive product inhibition and non-competitive prod-
uct inhibition.
(b) Explain the product inhibition on the product formation. [8+8]
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