ENZYME ENGINEERING AND TECHNOLOGY-SUPPLY2K6-RR

Code No: RR222303 Set No. 1
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. State the importance of enzymes in medical diagnosis. [16]
2. Describe the procedures of enzyme isolation from natural sources. [16]
3. Give an account of various factors that affect enzyme catalyzed reaction. [16]
4. Write the significance of MM equation. [16]
5. Explain various types of enzyme inhibition with relevant examples. [16]
6. Discuss the important criteria and prerequisite for selecting support for enzyme
immobilization. [16]
7. Discuss about the dynamic models of bioreactors. [16]
8. Discuss the design of enzyme electrodes. [16]
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Code No: RR222303 Set No. 2
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What are enzymes? Justify its role as biocatalysts. [16]
2. Describe the procedures of enzyme isolation from natural sources. [16]
3. Give an account of various factors that affect enzyme catalyzed reaction. [16]
4. Derive Michaelis-Menten equation. State the importance of MM constant. [16]
5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation.
[16]
6. Discuss about the interactions and carries used for enzyme immobilization by ad-
sorption. [16]
7. Discuss the design and application of packed bed reactors. [16]
8. What are the different types of transducer used in biosensors? [16]
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Code No: RR222303 Set No. 3
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Discuss the importance of enzymes in pharmaceutical industry? [16]
2. How are enzymes characterized? What are the importances of enzyme characteri-
zation? [16]
3. Discuss different types of enzyme specificity with suitable examples. [16]
4. Derive Michaelis-Menten equation. State the importance of MM constant. [16]
5. How MWC model account for cooperativity and explain allosteric regulation. [16]
6. Discuss the important criteria and prerequisite for selecting support for enzyme
immobilization. [16]
7. Give an account of different types of immobilized enzyme reactors. [16]
8. Enumerate the application of enzymes in analysis. [16]
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Code No: RR222303 Set No. 4
II B.Tech II Semester Supplementary Examinations, Aug/Sep 2006
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. State the importance of enzymes in medical diagnosis. [16]
2. How enzymes are preliminarily purified from a crude enzyme extract? [16]
3. Discuss why the lock & key model could lead to an inefficient enzyme mechanism
and induced fit model to an efficient enzyme mechanism. [16]
4. Discuss about the Eadie-Hofstee and Hanes plot and state their edge over the LB
plot. [16]
5. What are allosteric enzymes? Discuss their role in the regulation of a metabolic
pathway. [16]
6. Discuss the important criteria and prerequisite for selecting support for enzyme
immobilization. [16]
7. Give an account of different types of immobilized enzyme reactors. [16]
8. How enzyme electrodes are useful in health care industry as biosensor. [16]
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