ENZYME ENGINEERING AND TECHNOLOGY-REG RR-2K4

Code No.: RR-222303
II B.Tech II Semester Regular Examinations, April/May 2004
ENZYME ENGINEERING AND TECHNOLOGY
(BioTechnology)
Time: 3 hours Max. Marks: 80
Answer any FIVE questions
All questions carry equal marks
- - -
1. What are Enzymes? Give an account on the role of Enzymes in biological
systems.
2. Write an essay on production of enzymes from plants and animals.
3. Write a note on factors altering enzyme activity.
4. Discuss the general features of enzyme kinetics. What is Michaelis-Menton
equation?
5. Mention the different kinds of techniques that can be used for immobilization of
an enzyme.
6. How the kinetics of immobilized enzymes differ from that for the soluble
enzymes?
7. What is an ‘ideal enzyme reactor’? Discuss the various causes that influence the
performance of an enzyme reactor. What are ‘mass transfer’ effects ( as seen in
an enzyme reactor ) ?
8. Discuss different applications of enzymes in analytical field.
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Set. No.
1
Code No.: RR-222303
II B.Tech II Semester Regular Examinations, April/May 2004
ENZYME ENGINEERING AND TECHNOLOGY
(BioTechnology)
Time: 3 hours Max. Marks: 80
Answer any FIVE questions
All questions carry equal marks
- - -
1. What are the differences between the free and immobilized enzymes?
2. Give an account on purification of enzymes from different sources (plant, animal
and microbial source)?
3. Discuss about concentration of substrate on enzyme activity. Also make a note of
Lineweaver-Burke Plot.
4. Discuss the various parameters that are involved in the Michaelis-Menton
equation and their significance.
5. Discuss in detail technique involved for enzyme immobilization.
6. List all major factors involved for the differentiation of enzymes.
7. Explain the quantitative relationship between the substarate concentration and the
rate of an enzymatic reaction. What is the significance of Km value.
8. How would you design an enzyme electrode? Explain with a suitable example.
- - -
Set No.
2
Code No.: RR-222303
II B.Tech II Semester Regular Examinations, April/May 2004
ENZYME ENGINEERING AND TECHNOLOGY
(BioTechnology)
Time: 3 hours Max. Marks: 80
Answer any FIVE questions
All questions carry equal marks
- - -
1. Write an essay on the enzyme isolation and purification.
2. Discuss in detail various methods for characterization of enzymes.
3. What is an active site of an enzyme? How the active site involves in enzyme
substrate complex formation?
4. Describe graphically the different types of enzyme inhibition and explain their
nature.
5. Discuss 3 examples of application of immobilized enzymes. Mention the kind of
immobilization techniques used.
6. Discuss the kinetics and applications of immobilized enzymes.
7. Discuss the general design concentrations that are required for enzyme reactors.
Write down the salient features of the operational stability and optimization of
enzyme reactors.
8. Discuss the concept of biosensors. What are their applications in industry?
- - -
Set No.
3
Code No.: RR-222303
II B.Tech II Semester Regular Examinations, April/May 2004
ENZYME ENGINEERING AND TECHNOLOGY
(BioTechnology)
Time: 3 hours Max. Marks: 80
Answer any FIVE questions
All questions carry equal marks
- - -
1. Outline the steps that are involved in the isolation of an enzyme from a biological
system.
2. Outline steps involved in the development of enzymatic assays.
3. Explain the following:
(a) Specificity of enzyme action.
(b) Kinetics of single substrate reactions.
(c) Turnover number.
4. What are allosteric enzymes? How allosteric enzymes differ (in their kinetic
behavior) from normal enzymes?
5. Write the advantages and disadvantages of different immobilization techniques.
6. Write a note on calculation of effectiveness of immobilized enzymes.
7. Explain the following:
(a) Ideal reactors
(b) Reactor dynamics
(c) Enzyme deactivation.
8. What do you understand by the term biosensor? What are its applications in
healthcare and environment?
- - -
Set No.
4