ENZYME ENGINEERING AND TECHNOLOGY-REG 2K6-RR

Code No: RR222303 Set No. 1
II B.Tech II Semester Regular Examinations, Apr/May 2006
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Write about the application of enzymes in food industries. [16]
2. Describe the procedures of enzyme isolation from natural sources. [16]
3. Discuss different types of enzyme specificity with suitable examples. [16]
4. Derive the general rate equation of Alberty for multi-substrate enzyme catalyzed
reaction. [16]
5. Discuss allosteric enzymes in terms of cooperativity. [16]
6. Discuss the important criteria and prerequisite for selecting support for enzyme
immobilization. [16]
7. Discuss about the effect of mass transfer resistance in immobilized enzymes. [16]
8. Discuss the design of enzyme electrodes. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222303 Set No. 2
II B.Tech II Semester Regular Examinations, Apr/May 2006
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Discuss enzyme classification in terms of four-digit classification number? [16]
2. Describe the procedures of enzyme isolation from natural sources. [16]
3. Discuss why the lock & key model could lead to an inefficient enzyme mechanism
and induced fit model to an efficient enzyme mechanism. [16]
4. Derive the general rate equation of Alberty for multi-substrate enzyme catalyzed
reaction. [16]
5. Discuss and compare the non-competitive and uncompetitive inhibition based on
their kinetics. [16]
6. Discuss in detail about the across linking method for enzyme immobilization. [16]
7. Give an account of different types of immobilized enzyme reactors. [16]
8. How enzyme electrodes are useful in health care industry as biosensor. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222303 Set No. 3
II B.Tech II Semester Regular Examinations, Apr/May 2006
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Discuss enzyme classification in terms of four-digit classification number? [16]
2. Discuss the methods employed for isolating enzymes soluble in cytoplasm and sub
cellular organelles from animal, Plant and microbial sources. [16]
3. Enumerate Lock & Key model and Koshland’s Induced Fit model. [16]
4. Derive Michaelis-Menten equation. State the importance of MM constant. [16]
5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation.
[16]
6. Discuss about the medical application of immobilized enzymes. [16]
7. Give an account of different types of immobilized enzyme reactors. [16]
8. Enumerate the application of enzymes in analysis. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1
Code No: RR222303 Set No. 4
II B.Tech II Semester Regular Examinations, Apr/May 2006
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Enumerate the diagnostic importance of enzymes. [16]
2. Describe the procedures of enzyme isolation from natural sources. [16]
3. Discuss different types of enzyme specificity with suitable examples. [16]
4. Discuss the kinetics for reversible reactions. [16]
5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation.
[16]
6. Discuss the important criteria and prerequisite for selecting support for enzyme
immobilization. [16]
7. Discuss about the dynamic models of bioreactors. [16]
8. Enumerate the application of enzymes in analysis. [16]
⋆ ⋆ ⋆ ⋆ ⋆
1 of 1