METABOLIC ENGINEERING-REG 2K6

Code No: RR412311 Set No.1
IV B.Tech. I Semester Regular Examinations, November -2006
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Describe in detail the primary screening involved in strain selection with an exam-
ple. [16]
2. Write short notes on:
(a) Co-metabolism during bio-conversion.
(b) Concerted feed back regulation. [10+6]
3. Define bioconversion, and list out in detail the advantages of bio conversion in
pharmaceutical industry with suitable examples. [16]
4. What is enzyme inhibition and detail the various modes of enzyme inhibition? [16]
5. Compare and contrast indirect and direct fermentations citing amino acid synthesis
as an example. [16]
6. List out biotechnological applications of enzymes (ex:- Proteases, Amylases etc.)
produced by fermentation. [16]
7. Detail on (with examples)
(a) Active transport
(b) Group transport. [8+8]
8. List out the organisms involved in production of secondary metabolites with detail
on one secondary metabolite as an example. [16]
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Code No: RR412311 Set No.2
IV B.Tech. I Semester Regular Examinations, November -2006
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. How can Metabolic pathways be genetically controlled. Explain with 2 examples.
[16]
2. Write in detail on:
(a) Passive diffusion
(b) Isozymes. [8+8]
3. Explain gene regulation by Jacob-Monad model citing ‘Lac’ operon as example.
[16]
4. Detail how gene dosage is evaluated and how does gene dosage effect fermentation
process. [16]
5. Describe in detail the various modes of diffusion. [16]
6. Explain the Fermentation parameters involved in production of wine from yeast.
[16]
7. Distinguish and differentiate concerted feed back regulation and cumulative feed
back regulation with examples. [16]
8. How can Mutation effect the Enzyme production of an organism. [16]
? ? ? ? ?
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Code No: RR412311 Set No.3
IV B.Tech. I Semester Regular Examinations, November -2006
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. List out and describe the parameters involved in scale-up of Fermentation process
from pilot scale. [16]
2. Describe
(a) Gene dosage
(b) Amino acid regulation of RNA synthesis. [10+6]
3. Explain mixed OG sequential bioconversions with suitable examples. [16]
4. Describe catabolite repression with tryptophan operon as example. [16]
5. Define mutation and various modes of generating mutations in improving biotech-
nology for an organism. [16]
6. Describe induction and repression phenomena with E.coli as example. [16]
7. How can permeability of an organism be altered in focus with primary metabolites.
[16]
8. Write a detailed note on:
(a) Glucose effect
(b) Active transport. [8+8]
? ? ? ? ?
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Code No: RR412311 Set No.4
IV B.Tech. I Semester Regular Examinations, November -2006
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
? ? ? ? ?
1. Describe in detail the secondary screening involved in strain selection with an ex-
ample. [16]
2. Write a detailed note on:
(a) CAMP
(b) Feed back repression. [10+6]
3. Define bioconversion and describe the factors involved in bioconversion with an
example. [16]
4. What is antibiotic fermentation and explain it’s methodology in pencillin produc-
tion. [16]
5. List out and detail the factors contributing to catalytic efficiency of Enzymes. [16]
6. How do mutations effect the enzyme production. List out the aspects involved in
optimization of mutants for high yield protein production. [16]
7. Describe in detail the conversion of insoluble substances by mixed or sequential
bioconversions. [16]
8. Write a detailed note on:
(a) Fed-batch fermentation
(b) Continuous fermentation. [8+8]
? ? ? ? ?
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