DOWN STREAM PROCESSING-SUPPLY-2K6

Code No: RR412302 Set No. 1
IV B.Tech I Semester Supplementary Examinations, March 2006
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Describe about Vitamins, Alkaloids, and Nucleosides of commercial interest? [16]
2. Explain the role of Downstream processing in Biotechnology? [16]
3. How can you classify Cell disruption methods? Explain in brief about them? [16]
4. Write short notes on:
(a) Tubular Centrifuge
(b) Multi chamber centrifuge [8+8]
5. Write about different modes of operation of filters? [16]
6. Outline the advantages and disadvantages three basic types of precipitation reac-
tions, batch reactor, CSTR and tubular reactor? [16]
7. Describe the following:
(a) Liquid adsorption chromatography
(b) LLC [8+8]
8. Write short notes on:
(a) Secondary nucleation
(b) Heterogeneous nucleation [8+8]
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Code No: RR412302 Set No. 2
IV B.Tech I Semester Supplementary Examinations, March 2006
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Examine the continuing role which economics plays in Bioprocess research,development
and commercialization? [16]
2. Explain about the primary isolation techniques? [16]
3. Describe about Mechanical methods of Cell disruption? [16]
4. Describe about Membrane Filter press with a neat sketch? [16]
5. Write about different modes of operation of filters? [16]
6. Describe about different techniques employed in Precipitation? [16]
7. Write short notes on:
(a) Paper chromatography
(b) SDS-PAGE [8+8]
8. Outline the strategies for Crystallization of proteins? [16]
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Code No: RR412302 Set No. 3
IV B.Tech I Semester Supplementary Examinations, March 2006
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Describe about economic assessment of various proteins via r DNA? [16]
2. Explain about the primary isolation techniques? [16]
3. How can you classify Cell disruption methods? Explain in brief about them? [16]
4. Explain about different types of Filter media and types of Filters used in Biotech-
nology industries? [16]
5. Write short notes on:
(a) Liquid membranes
(b) Ultra filtration [8+8]
6. Describe about different techniques employed in Precipitation? [16]
7. Explain about different types of Adsorbents employed in Adsorption process?[16]
8. Explain about the process of Dialysis? [16]
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Code No: RR412302 Set No. 4
IV B.Tech I Semester Supplementary Examinations, March 2006
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Examine the continuing role which economics plays in Bioprocess research,development
and commercialization? [16]
2. Explain the principle of Solvent extraction and its applications with an example?
[16]
3. Write in detail about physico-mechnaical methods of Cell disruption? [16]
4. Explain about different types of Filter media and types of Filters used in Biotech-
nology industries? [16]
5. Explain and describe the following membrane separation process Dialysis and Liq-
uid membranes? [16]
6. Explain in detail about extraction fundamentals? [16]
7. Explain about different Chromatography employed for separation of complex mix-
tures? [16]
8. Explain about the process of Dialysis? [16]
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