DOWN STREAM PROCESSING-REG 2K7

Code No: RR412302 Set No. 1
IV B.Tech I Semester Regular Examinations, November 2007
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Describe about Vitamins, Alkaloids, and Nucleosides of commercial interest? [16]
2. Explain about different ways of precipitation and its applications. [16]
3. Write short notes on:
(a) Enzymatic cell lysis
(b) Chemical cell lysis [8+8]
4. Write about the Pressure leaf Filters? [16]
5. Write short notes on:
(a) Liquid membranes
(b) Reverse Osmosis [8+8]
6. Write short notes on:
(a) whole broth treatment
(b) in-situ product recovery [8+8]
7. Describe about different types of support media employed in electrophoresis? [16]
8. Explain the Formation and growth of Crystals? [16]
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Code No: RR412302 Set No. 2
IV B.Tech I Semester Regular Examinations, November 2007
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What are the various products of r DNA and their economic assessment of various
proteins? [16]
2. Describe about extraction using aqueous two phase systems? [16]
3. How can you classify Cell disruption methods? Explain in brief about them? [16]
4. Explain about different types of Filter media and types of Filters used in Biotech-
nology industries? [16]
5. Write about different modes of operation of filters? [16]
6. Describe about different agents employed in precipitation which renders the com-
pounds of interest insoluble? [16]
7. Describe about Chromatographic columns and column packing procedures? [16]
8. Explain about the process of Dialysis? [16]
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Code No: RR412302 Set No. 3
IV B.Tech I Semester Regular Examinations, November 2007
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Design a material balance sheet for certain process and give its capital investment
estimate for the major equipment of the process in material balance sheet? [16]
2. Explain the principle of Solvent extraction and its applications with an example?
[16]
3. Describe about Mechanical methods of Cell disruption? [16]
4. Write short notes on:
(a) Basket Centrifuge
(b) Solid bowl scroll Centrifuge [8+8]
5. Write about different modes of operation of filters? [16]
6. Describe about different techniques employed in Precipitation? [16]
7. Describe in detail about adsorptive chromatographic separation process? [16]
8. Describe about Crystallization process of proteins? [16]
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Code No: RR412302 Set No. 4
IV B.Tech I Semester Regular Examinations, November 2007
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Examine the continuing role which economics plays in Bioprocess research,development
and commercialization? [16]
2. Give generalized process recovery scheme for enzymes derived form animal, plant,
surface or submerged fermentations? [16]
3. How can you classify Cell disruption methods? Explain in brief about them? [16]
4. What are the various techniques employed in recovery of Bioproducts? Explain in
brief about them? [16]
5. Compare and contrast different modes of filtration process? [16]
6. Describe the importance of process integration and whole broth treatment and
in-situ recovery strategies for product recovery? [16]
7. Write short notes on:
(a) Paper chromatography
(b) SDS-PAGE [8+8]
8. Explain about the process of Dialysis? [16]
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