DOWN STREAM PROCESSING-REG 2K6

Code No: RR412302 Set No. 1
IV B.Tech I Semester Regular Examinations, November 2006
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Examine the continuing role which economics plays in Bioprocess research,development
and commercialization? [16]
2. Describe about extraction using aqueous two phase systems? [16]
3. Explain about working of a bead mill and on what factors does the optimal condi-
tions in a Bead mill depends upon? [16]
4. Write short notes on:
(a) Basket Centrifuge
(b) Solid bowl scroll Centrifuge [8+8]
5. What are the different types of Membranes available for separations and explain
about different membrane configurations? [16]
6. Explain the concept of DLVO theory? [16]
7. Explain about the basic concepts of Electrophoresis? [16]
8. Explain about the process of Dialysis? [16]
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Code No: RR412302 Set No. 2
IV B.Tech I Semester Regular Examinations, November 2006
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Describe about economic assessment of various proteins via r DNA? [16]
2. Give generalized process recovery scheme for enzymes derived form animal, plant,
surface or submerged fermentations? [16]
3. How can you classify Cell disruption methods? Explain in brief about them? [16]
4. Write about the Pressure leaf Filters? [16]
5. How can RO and Ultra filtration complement each other? [16]
6. Describe about different techniques employed in Precipitation? [16]
7. Write short notes on:
(a) Distribution coefficients
(b) Modes of chromatography [8+8]
8. Explain about the process of Dialysis? [16]
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Code No: RR412302 Set No. 3
IV B.Tech I Semester Regular Examinations, November 2006
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Examine the continuing role which economics plays in Bioprocess research,development
and commercialization? [16]
2. Explain about the Filtration technique employed for several Bioproducts? [16]
3. What is meant by cell disruption and explain about the functioning of Bead mill?
[16]
4. Explain about Cell aggregation and Flocculation? [16]
5. Write short notes on:
(a) Liquid membranes
(b) Ultra filtration [8+8]
6. Describe about different methods for extraction of Bioproducts? [16]
7. Derive the mass balance for fixed bed adsorption? [16]
8. Explain and classify the types of Crystallization used based on how supersaturation
is achieved? [16]
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Code No: RR412302 Set No. 4
IV B.Tech I Semester Regular Examinations, November 2006
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Describe about preliminary economic analysis or cost analysis performed in Bio-
process? [16]
2. Explain about the Filtration technique employed for several Bioproducts? [16]
3. Write short notes on:
(a) Enzymatic cell lysis
(b) Chemical cell lysis [8+8]
4. What is the difference between sedimentation and Centrifugation and List the ad-
vantages and disadvantages of Centrifugation? [16]
5. Write about different modes of operation of filters? [16]
6. Describe about different techniques employed in Precipitation? [16]
7. Explain about Affinity electrophoresis and Immuno electrophoresis? [16]
8. Describe about the process of Electro Dialysis? [16]
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