MICROBIOLOGY supply 2k9

Code No: 43113 Set No. 1
II B.Tech I Semester Supplimentary Examinations, May/Jun 2009
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Write the principle and method involved in AFB staining. [16]
2. Write about five kingdom classification of R.H.Whittaker. [16]
3. Describe the structure, composition and multiplication of HIV. [16]
4. Describe in detail the various steps involved in replication of lysogenic /temperate
phage with illustration. [16]
5. (a) Describe methods of cultivation of viruses.
(b) How do you quantify viruses? [8+8]
6. (a) Elaborate the differential media and discuss its role in the isolation of microor-
ganism.
(b) What is an enrichment media and how it is useful in culturing microorganisms.
[8+8]
7. Mention reasons behind gram positivity of bacteria. [16]
8. Explain Mycobacterium tuberculosis’s:
(a) Morphology
(b) Toxin production
(c) Pathogenicity
(d) Diagnosis [4×4]
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Code No: 43113 Set No. 2
II B.Tech I Semester Supplimentary Examinations, May/Jun 2009
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Write about contributions of Louis Pasteur emphasizing on Biogenesis theory? [16]
2. Describe the structure of prokaryotic cell and relate it to its function. [16]
3. Define viruses and write about general properties of viruses? [16]
4. Describe and illustrate the different steps involved in replication of lytic phage.
[16]
5. (a) Describe the biological assay methods for animal viruses.
(b) Describe the methods involved in the identification of viruses. [8+8]
6. Write short notes on :
(a) Endospore forming bacteria.
(b) Nonspore forming bacteria. [8+8]
7. What are:
(a) Bacterial spores and
(b) Elaborate the spore staining technique. [8+8]
8. Write briefly on:
(a) Preparation of active immunization against Diphtheria.
(b) Passive and combined immunization against Diphtheria.
(c) Treatment for Diphtheria. [8+4+4]
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Code No: 43113 Set No. 3
II B.Tech I Semester Supplimentary Examinations, May/Jun 2009
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Ecplain different staining proceducres. [16]
2. Give the names and main distinguishing characteristics of the five kingdoms in
Whittaker‘s classification. [16]
3. What are viroids? How does a prion differ from virus? Write the pathogenicity of
prions? [16]
4. Explain the replication mechanism of TMV. [16]
5. Write about:
(a) Assay for bacterial viruses.
(b) Assay for animal viruses. [8+8]
6. (a) Discuss different types of solidifying agents and their uses in preparation of
media.
(b) How a suitable culture media is prepared for culturing the bacteria? [8+8]
7. (a) What are primary stock and working cultures?
(b) Explain about subculturing. [8+8]
8. Write briefly on:
(a) Pathogenicity
(b) Laboratory diagnosis
(c) Prophylaxis treatment for disease caused by Staphylococci bacteria. [8+4+4]
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Code No: 43113 Set No. 4
II B.Tech I Semester Supplimentary Examinations, May/Jun 2009
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Ecplain different staining proceducres. [16]
2. Compare and contrast between prokaryotic and eukaryotic cell structures. [16]
3. Define viruses and write about general properties of viruses? [16]
4. Explain the replication mechanism of ds DNA lytic phage. [16]
5. Write notes on:
(a) In vivo virus cultivation
(b) In vitro virus cultivation. [8+8]
6. Write short notes on the following :
(a) Oxygen toxicity
(b) Anaerobic chambers and Anaerobic work stations. [8+8]
7. What are:
(a) Bacterial spores and
(b) Elaborate the spore staining technique. [8+8]
8. Write short notes on:
(a) Hepatitis-A viruses
(b) Hepatitis-B viruses. [8+8]
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