ENZYME ENGINEERING AND TECHNOLOGY-SUPPLY2K7-RR

Code No: RR222303 Set No. 1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. State the importance of enzymes in medical diagnosis. [16]
2. How enzymes are preliminarily purified from a crude enzyme extract? [16]
3. Give an account of various factors that affect enzyme catalyzed reaction. [16]
4. Derive the general rate equation of Alberty for multi-substrate enzyme catalyzed
reaction. [16]
5. Explain the kinetics of competitive inhibition. Add a note on its importance. [16]
6. Write the merits and demerits of enzyme immobilization techniques. [16]
7. Give an account of different types of immobilized enzyme reactors. [16]
8. What are the different types of transducer used in biosensors? [16]
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Code No: RR222303 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. State the importance of coenzymes and cofactors in enzyme catalysis. [16]
2. Describe the procedures of enzyme isolation from natural sources. [16]
3. Distinguish between the lock & key and Induced Fit models for binding of a substrate
to an enzyme. [16]
4. Discuss about the Eadie-Hofstee and Hanes plot and state their edge over the LB
plot. [16]
5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation.
[16]
6. Discuss the important criteria and prerequisite for selecting support for enzyme
immobilization. [16]
7. Enumerate the kinetics of immobilized enzymes. [16]
8. Enumerate the application of enzymes in analysis. [16]
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Code No: RR222303 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What are enzymes? Justify its role as biocatalysts. [16]
2. Discuss the methods employed for isolating enzymes soluble in cytoplasm and sub
cellular organelles from animal, Plant and microbial sources. [16]
3. Distinguish between the lock & key and Induced Fit models for binding of a sub-
strate to an enzyme. [16]
4. Discuss the kinetics for reversible reactions. [16]
5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation.
[16]
6. Give an account of analytical application of immobilized enzymes. [16]
7. Formulate and calculate dimensionless groups and effectiveness factor. [16]
8. Enumerate the application of enzymes in analysis. [16]
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Code No: RR222303 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
ENZYME ENGINEERING AND TECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Discuss enzyme classification in terms of four-digit classification number? [16]
2. Describe the procedures of enzyme isolation from natural sources. [16]
3. Explain the energetics of enzyme-substrate complex formation. [16]
4. Derive Michaelis-Menten equation. State the importance of MM constant. [16]
5. Explain various types of enzyme inhibition with relevant examples. [16]
6. Discuss the important criteria and prerequisite for selecting support for enzyme
immobilization. [16]
7. Give an account of different types of immobilized enzyme reactors. [16]
8. Enumerate the application of enzymes in analysis. [16]
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